Stabilization of Molecular Structure of the Proteins and Lipids of the Erythrocyte Membranes by Means Magnetite Nanoparticles (ICNB)

Andrey Belousov1,2,3*, Elena Malygon2,3, Vadim Yavorskiy2,3, Ekateryna Belousova1
*Corresponding author
Andrey Belousov, Laboratory of Applied Nanotechnology of Belousov, Ukraine, Web site: www.nanolab.com.ua, E-mail: an.belousov2012@ukr.net
Submitted: 16 Jan 2019; Accepted: 25 Jan 2019; Published: 05 Feb 2019
1Laboratory of Applied Nanotechnology of Belousov (UA)
2Kharkov Medical Academy of Postgraduate Education (UA)
3Kharkov Regional Center of Blood Service (UA)

Abstract
This study was devoted to the learning changes in the structure of erythrocyte membranes at the level of molecular bonds during their storage at a positive temperature by means method of infrared spectroscopy (IR).Objects of research were red blood cells (RBCs) into bags containing preservative CPD and RBCs into bags containing preservative CPDA-1.As membrane protective used saline which had previously been treated with magnetite nanoparticles (ICNB) by the Belousov’s method. The physiological solution that was treated with nanoparticles was added to the preserved RBCs according to the developed method. Sample of control was the addition of intact saline. Analysis of changes occurring in the IR spectra of samples of control and test in the CPD medium was showed that during the first 28 days storage of: 1. In the suspension of control of the RBCs, noticeable destructive changes in the molecular structure membrane of erythrocytes at the 14th day storage begin. After three weeks, the molecular structure membrane of erythrocytes is completely destroyed; 2. In the sample of test there was a weakening and rupture of molecular bonds only at the 28th day storage of RBCs. Complete destruction of the structure of membrane of erythrocytes occurs at the 35th day storage. Analysis of changes occurring in the IR spectra of samples of control and test in the CPDA-1 medium was showed that during 49 days storage of …

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